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Saulėtekio al. 11, SRL-I, 203 room |
Head of a department: prof.habil. dr. Juozas Kulys |
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Current Research Projects 1. Kinetic analysis of synergistic enzymatics reactions. Supervisor: J. Kulys Kinetics of biocatalytical synergistic reactions has been analyzed at non-stationary and at quasi steady-state conditions. The expression for quasi steady-state was derived using symbolic algebra under assumption of irreversibility of all reactions. Experimentally the synergistic reactions were investigated using 3 pairs of substrates. 2. Isolation and putification of recombinant growth hormones. Supervisor: V. A. Bumelis Recombinant porcine growth hormone was purified from E. coli inclusion bodies. Steps of recovery of highly purified and bioactive protein are as follows: solubilization of inclusion bodies, refolding of target protein and chromatography on Q-Sepharose and Phenyl-Sepharose. The purity of protein was tested using reverse-phase high performance liquid chromatography and sodium dodecyl sulphate polyacrylamide gel electrophoresis under nonreducing and reducing conditions. The method of recombinant porcine growth hormone purification was also applied for recovery of mink growth hormone. 3. Investigation of long-term conservation of drinking water preparation equipment. Supervisor: J. Jankauskas This study had a clear advantage of being conducted using real industrial water preparation equipment rather then experimental filter models. I thank Joint Stock company Anykščių vandenys for this possibility. Two filter conservation ways were tested: a) Filter was rinsed, put to work and immediately stopped. Filtration bed was left under water. Filter was kept idle for 12 months. Chemical analysis of filter water was conducted periodically. Its results revealed chemical and microbiological processes inside the filtration bed. b) Filter was rinsed and stopped removing water from it. Filtration bed was left wet. This filter was idle for 12 months as well. Filters a) and b) were rinsed and put to work after 12 months. Periodical chemical analysis of filtered water allowed to answer the key question: how long it would take for the filters to recover previous efficiency of iron, ammonia and manganese removal. Filter a) had recovered initial efficiency in 72 hours (iron and ammonia concentration in filtered water had not exceeded the hygiene norms in 4 h, manganese in 72 h). Same sequence of removal was left: iron was removed first, followed by ammonia and finally manganese. By comparison, brand new filters would effectively start to remove iron in 1-2 days, ammonia in 1-3 months and manganese in 3 to 6 months. Indicated timeframe may vary depending on the quality of row water. Even better results were obtained in test b), when filter was left without water. Excellent results were possible after only 3 hours of restarted exploitation .
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